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While none of the mutations tested induced obvious morphological changes or migratory abilities, clear differences were identified in the induction of EGF-independent proliferation and anchorage-independent growth.

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Most biochemical markers in CSF have poor sensitivity and specificity, but when present, levels decline with successful therapy.

Janmaat ML, Kruyt FA, Rodriguez JA, Giaccone G Response to epidermal growth factor receptor inhibitors in non-small cell lung cancer cells: Limited antiproliferative effects and absence of apoptosis associated with persistent activity of extracellular signal-regulated kinase or Akt kinase pathways.

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Treatment Rationale and Study Design for the RELAY Study: A Multicenter, Randomized, Double-blind Study of Erlotinib plus Ramucirumab or Placebo in Patients with Epidermal Growth Factor Receptor Mutation-positive Metastatic Non-Small Cell Lung Cancer.

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Enzymes lower the activation energy of reactions by bringing reactants closer to each other on the active site, rather than relying on collisions between them to produce the product.

Instead, L858R-T790M-EGFR had increased affinity to ATP when compared with L858R alone, which is predicted to decrease binding of gefitinib and erlotinib because these drugs are ATP-competitive kinase inhibitors.

Combined analysis of molecular and clinical predictors of gefitinib activity in advanced non-small cell lung cancer: epidermal growth factor receptor mutations do not tell the whole story.

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A549 cells were treated with gefitinib (10 μM) or erlotinib (10 μM) for indicated times and tunicamycin (0.

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We next tested if overexpression of EGFR could induce FOXM1 expression.

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No pemetrexed-related deaths occurred.

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Chinese patients with advanced NSCLC participating in the compassionate-use programme at Peking Union Medical College Hospital.

The results of an EGFR-silencing study using siRNA showed that gefitinib and erlotinib affected eIF2α phosphorylation and cyclin-D1 expression independent of EGFR inhibition.

The application of the preclinical PD pERK model to patients was done without any variable scaling to account for possible differences between mouse xenograft gliomas and patient tumors.

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Possible pharmacokinetic interaction (decreased plasma gefitinib concentrations, possible reduction in gefitinib efficacy) with drugs that cause substantial, sustained gastric pH elevation.

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We also did not find a suitable nonlinear PD model for gefitinib but did find functional relationships between both IC 50 and K tr and the AUC t (see Supplementary Fig.

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Matched normal tissue was available for Patients 1, 4, 5, and 6 and showed only the wild-type sequence, indicating that the mutations had arisen somatically during tumor formation.

Concurrence of EGFR amplification and sensitizing mutations indicate a better survival benefit from EGFR-TKI therapy in lung adenocarcinoma patients.

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Non-small-cell lung cancer (NSCLC) accounts for approximately 80-85% of all cases of lung cancer, and is the most common cause of death in men and second only to breast cancer in woman ().

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The pertinent PD variables in the target-response model are K in, K out, K tr, and IC 50, and how these may be related between species will have to be addressed in a broader context with more patient data.

Due to progressive disease revealed in September 2012, systemic therapy was changed to pemetrexed and continued after achieving stable disease in December 2012.

Afatinib versus erlotinib as second-line treatment of patients with advanced squamous cell carcinoma of the lung (LUX-Lung 8): an open-label randomised controlled phase 3 trial.

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Gefitinib sensitivity was restored in nuclear PKM2-overexpressed cells after siRNA knockdown of STAT3, according to an MTS assay, suggesting that nuclear PKM2-induced gefitinib resistance was dependent on STAT3 ().

This indicated the weak induction of G 1 arrest of cell cycle in HER2-overexpressing GLM-1 cells by gefitinib.

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Evidence of a modest increase in survival in patients with better PS given high-dose EBRT is available.

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